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Beginning of the lesson

Protein primary sequence, alpha helix, beta sheet, tertiary fold, four-subunit assembly, and unfolding without backbone cleavage
The hierarchy of protein structure and denaturation

Proteins: sequence, shape, and function

Proteins are polypeptides whose amino-acid sequences fold into specific three-dimensional structures. A protein’s function depends on both its sequence and its environment. Peptide bonds build the backbone; many weaker interactions organize the fold.

Learning goals

  • describe primary, secondary, tertiary, and quaternary structure;
  • identify forces stabilizing proteins;
  • connect shape to biological function;
  • distinguish denaturation from hydrolysis;
  • interpret a general peptide-bond test.

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  • Worked examples
  • Common mistakes and how to avoid them
  • Test questions: 15
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